For labelling experiments, cells were seeded as described above. Then cells were changed to NSMM, with 50% 13C-glucose for labelling. The glucose was isotopically labelled at all six carbon positions ([U- 13C6] glucose). After 24 h of incubation, labelled cells were rinsed with cold PBS. Cells were then processed for GC/MS sample extraction and derivatization, and analysed as described before5354.
