Withdrawal of doxycycline at any time point had no significant effect on MAP2AB signal intensity at three weeks of culture (Fig. 5B and C); quantified across biological triplicates from one NPC line. Moreover, no statistically significant difference in the number of SYN1-positive puncta was observed (Fig. 5D and E); quantified across biological triplicates from one NPC line. Strikingly, doxycycline addition for as little as two days was sufficient to increase expression of the synaptic genes SYN1, vGLUT1 and vGLUT2 relative to GFP-transduced NPCs (Fig. 5F); quantified across biological triplicates from one NPC line.
