behavior which may correspond to the ability to tile the CNS by their in vivo counterparts. Unlike other tissue-resident macrophages, microglia derive from PU.1+/IRF8+/MYB− precursors 34,35. Expression of the same markers in pMGLs supports this ontogeny. pMGLs kept in mono-culture express high levels of SELPLG, TXNIP, CST3 and OLFML3, suggesting they adopt a relatively more mature profile than primary fetal cells.
