and/or αβ neuron activation is required for the acute locomotor stimulatory effects of ethanol39, suggesting that the acute response to the ethanol stimulus requires mushroom body neuron activation. Our results, however, must be interpreted with caution since we did not use a γ neuron-specific driver. It is possible that acquisition of memory may require non-overlapping subsets of αβ neurons defined by the 5-66a, 201Y, and MB247 GAL4 drivers.