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Chunk #34 — Materials and Methods — Integration of the Illumina H8V2 and HT12 peripheral blood expression platform identifiers

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Trans-eQTLs reveal that independent genetic variants associated with a complex phenotype converge on intermediate genes, with a major role for the HLA.
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The HT12 and H8v2 arrays share a considerable number of probes with identical probe sequences. However, in a considerable number of occasions the two platforms use different probe identifiers for the same probe sequences. More importantly, although probe identifiers are often identical, they sometimes represent different probe sequences. In order to permit a meta-analysis incorporating data from both arrays, we decided on the following naming convention: if an H8v2 probe had the same sequence as an HT12 probe, the HT12 ‘ArrayAddressID’ probe identifier was used. If not, the original H8v2 probe identifier was used, but with the prefix “Human_RefSeq-8_v2-” to prevent any potential probe identifier ambiguity. A total of 52,061 unique probes were used for further analysis, representing 19,609 unique genes according to HUGO gene nomenclature.