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Chunk #13 — Materials and Methods — Genotyping

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Examination of genetic variation in GABRA2 with conduct disorder and alcohol abuse and dependence in a longitudinal study.
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SNPs in the CADD and GADD, respectively, are shown as follows: rs567926 (0.78%, 0.00%), rs279858 (0.78%, 0.26%), rs279871 (0.52%, N/A), rs279845 (1.30%, 0.00%), rs9291283 (0.26%, 0.78%). Genotype clusters from the amplified 384 well plates were auto-called by the Applied Biosystems TaqMan® Genotyper software (Applied Biosystems, Foster City, California) and subsequently visually examined by two independent lab personnel to verify calls. Final calls were determined when both laboratory personnel agreed; if they did not agree genotypic data were excluded.