immune subtypes. ZDHHC5 was slightly downregulated in C3 and was overexpressed in C4 (Figure 2C), which is in accordance with the proposed malignant role of ZDHHC5 in LUAD. We also explored the Spearman correlation of ZDHHC5 with 3 types of immune regulators and tumor-infiltrating lymphocytes in LUAD, but there was no significant association (Supplementary Figure 1A). TCGA Consortium proposed a transcriptional data-based molecular classification for LUAD, which includes 3 molecular subgroups into the clinical classification of LUAD (Cancer Genome Atlas Research Network, 2014). We performed gene expression analysis of ZDHHC5 in the 3 molecular subtypes (Supplementary Figure 1B), but there was no significant difference (P > 0.05). As shown in Figure 2D, the mRNA expression level of ZDHHC5 was higher in TP53-mutant LUAD cases than in the non-mutant cases.
