number of GIRK3 immunoparticles per synapse and percentage of synapses labelled at all ages studied (P5, 1.47 ± 0.11 immunoparticles/labelled synapse; P15, 1.53 ± 0.11 immunoparticles/labelled synapse; P60, 1.54 ± 0.12 immunoparticles/labelled synapse; Fig. 7G-I, Table 2). We used anti-PSD-95 and anti-GluN1 antibodies as controls for postembedding experiments at each developmental age and always found three to four times more immunoparticles (not shown) than those for anti-GIRK antibodies, suggesting no technical limitations.
