antibodies. Then, cells were incubated with the appropriate secondary antibodies conjugated with fluorophores, examined and imaged using the confocal microscope (Leica TCS SP5 Confocal Laser Scanning Microscope). All antibodies were commercially available: Tau antibody BT-2, pTau181 antibody AT270, and PAX6 antibody were purchased from ThermoFisher (Waltham, MA); Nestin, Sox1, Sox2, Glial Fibrillary Acidic Protein (GFAP), NeuN, β tubulin III (BT3), and microtubule-associated protein 2 (MAP2) antibodies were purchased from EMD Millipore (Billerica, MA).
