was no opportunity for replication of the individual novel variants. Because the variants were detected in more than 430,000 subjects and have small effect sizes, a replication sample with adequate power would also have to be very large, and no such sample is currently available. To validate the findings, we conducted PRS analyses in three independent cohorts, which showed strong association with AUD. Although this indicates that our study had adequate power for variant detection, it does not address the validity of the individual variants discovered.
