lower anti-inflammatory interleukin IL-4 (T(27.1) = 4.91, P = 0.00004) and IL-10 (T(29.8) = 2.84, P = 0.008) and lower chemokine IL-8 (T(26) = 3.69, P = 0.001) (see [32] for details). From these subjects (sample 1), the methylation analysis of these cytokine gene loci and their transcription factors was done on all the 8 subjects from the CPA group and a representative subgroup of 12 subjects was selected for the control group (sample 2). Note that one CPA subject was removed from the ELISA analysis due to cytokine concentrations outside of the expected range for the standard curve but was included in the methylation analysis. A schematic overview of the recruitment is illustrated in Figure S7. Characteristics of the original samples (n = 32 sample 1) and the selected subgroups (n = 20 sample 2) are presented in Table 3.
