Chunk #91 — Results and discussion — Analysis of Akt/mTOR phosphoprotein pathway throughout the brain after chronic alcohol: Decreased expression of multiple mTOR/Akt phosphoproteins in 3xTg-AD alcohol-exposed mice (1-month post alcohol) — Hippocampus
In three subregions of the HPC, (medial, lateral, and CA1) analyzed in this study, the effects on the mTOR/Akt pathway were subregion specific. This is an important distinction from other researchers looking at hippocampal changes as the subregion is not often specified. In the MHPC, no significant changes were observed in the expression of Akt/mTOR phosphoproteins between alcohol and saccharin exposed 3xTg-AD mice (Table 3). In the LHPC, a history of alcohol drinking in 3xTg-AD mice was associated with significant reductions in phosphorylation of IRS1 [t(6) = 1.974, P = 0.048] and P70S6K [t(6) = 2.078, P = 0.042] as compared to saccharin controls (Table 3). Finally, in CA1, a history of chronic alcohol drinking in 3xTg-AD mice was associated with a significant reduction in phosphorylated mTOR [t(7) = 3.011, P = 0.01] and PTEN [t(7) = 3.021, P = 0.01] as compared to saccharin controls (Table 3).
