▲ CRITICAL STEP Note that the MgCl2 used for SURVEYOR nuclease digestion (included in the SURVEYOR mutation detection kit) is a higher concentration from that used for SURVEYOR PCR. 82Vortex the mixture well and spin it down briefly. Incubate the reaction at 42 °C for 30 min.83(Optional) Add 2 μl of the Stop Solution from the SURVEYOR kit if you do not intend to visualize the reaction products (next step) immediately.
