Chunk #9 — MATERIALS AND METHODS — Immunofluorescence assays

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Neurons derived from patients with bipolar disorder divide into intrinsically different sub-populations of neurons, predicting the patients' responsiveness to lithium.

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The antibodies used for pluripotency and neuronal characterization were anti-hOct4 (Cell Signaling, Danvers, MA, USA; cat. no. 2840S), anti-hSox2 (Cell Signaling; cat. no. 3579S), anti-hNanog antibody (Cell Signaling; cat. no. 4903S), Tra-1-81 (Cell Signaling; cat. no. 4745S), anti-MAP2 (Abcam; cat. no. 5392) and anti-GFP (Abcam, San Francisco, CA, USA; cat. no. 6673).