Chunk #17 — Results — Alcohol stimulates expression of the EMT signature mesenchymal marker protein vimentin in breast and colon cancer cells and normal intestinal epithelial cells

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Alcohol stimulates activation of Snail, epidermal growth factor receptor signaling, and biomarkers of epithelial-mesenchymal transition in colon and breast cancer cells.

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(81 ± 4) and alcohol treated (157 ± 6). Thus, the untreated Caco-2, MCF-7, and IEC-6 cells showed low vimentin expression. However, the MDA-MB-231 cells did show higher basal expression of vimentin. High basal expression of vimentin in the MDA-MB-231 cells was also noted by others(Nagaraja et al., 2006). Nonetheless, alcohol stimulated a significant increase in vimentin staining in all four cell types (Figure 1A–D; p ≤ .05; control vs. alcohol treated t-tests).