Only those SNPs on the genotyping array with an alternative allele frequency of >5% (N = 61 731) were included in the analysis. Total genetic distance among samples was calculated and plotted (Fig. 2). Genetic distances in the selected lines were relatively constrained when compared with the HS-CC founder strains. Strong divergence between the high and low preferring lines was observed. From a genetic distance perspective, the High line was closest to the WSB/EiJ inbred strain. Note that considerable genetic diversity was maintained in the selected lines.
