Examining the relationship between XIST expression and X chromosome DNA methylation, we noted that there was a relative threshold of XIST expression, above which we saw uniform partial DNA methylation, and below which we observed decreased methylation in at least a subset of CpG sites (Figure 5C). This result was consistent with DNA methylation on the chromosomal level (Figure 5B), where it was apparent that the absence of DNA methylation on the X chromosome occurred in a patchy fashion.
