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Chunk #6 — Materials and Methods — Simple Sequence Repeat Genotyping

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Fine mapping of calcineurin (PPP3CA) gene reveals novel alternative splicing patterns, association of 5'UTR trinucleotide repeat with addiction vulnerability, and differential isoform expression in Alzheimer's disease.
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Simple sequence length polymorphisms were identified throughout the PPP3CA gene by alignment of genomic with expressed sequence tag (EST) and mRNA sequences available from NCBI (www.ncbi.nlm.nih.gov) using Sequencher software (Version 3.0, Gene Code Co., Ann Arbor, MI).