Chunk #33 — Materials and methods — Bioinformatics analysis of cross-species ranked gene list

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Multi-species data integration and gene ranking enrich significant results in an alcoholism genome-wide association study.

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The 47 top ranked genes with a score ≥ 2 were examined for enriched GO terms using the WebGestalt online tool (version 2) [32]. This tool examines the over-representation of genes of interest in GO terms based on the hypergeometric test followed by the Benjamini-Hochberg (1995) adjustment of p-values [50]. We then used the ToppFun online tool [57], which is an integrated over-representation analysis tool, to interrogate databases for biological pathways, mouse phenotypes, and PubMed citations.