In our experimental conditions, LPHN3-OLF crystallized in a hexagonal (P65) or an orthorhombic (C2221) lattice. The structure was solved by native-SAD phasing using the recently described multi-crystal phasing procedure (Liu et al., 2012; Liu et al., 2014) with the P65 crystal form. The resulting initial model was then used to position the LPHN3-OLF in the C2221 lattice by molecular replacement and both structures were completed and refined using 1.6 Å high resolution datasets (see Methods, Table 2).
