PCR primers for the amplification of enhancer and control regions were designed using the PerlPrimer tool45, and purchased from Operon Ltd. Primers included BamHI or SalI restriction sites for cloning and sequences are listed in Supplementary Tables 2 and 3. Control fragments ranged between 420-1452bp. Enhancer fragments usually included a 500bp window around the mid point of our predicted enhancers and depending on the availability of unique primer sequences, enhancer fragments ranged between 470-840bp.
