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Chunk #11 — MATERIAL AND METHODS — Genotyping methods and biochemical analysis

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Genetic polymorphisms in 15q25 and 19q13 loci, cotinine levels, and risk of lung cancer in EPIC.
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Cases and controls were randomly mixed when genotyped and laboratory staff were blinded to case-control status. A random selection of 5% of the study subjects were genotyped twice for quality control. Genotyping success rate per SNP in the present study ranged between 93% and 100%. Internal duplicate concordance was >98.7% for all variants. All variants showed genotype distributions consistent with that expected under Hardy-Weinberg equilibrium (HWE) using a P threshold of 0.005 (Bonferroni correction for 10 tests).