We then induced AD-NPCs and N-NPCs into neurons and examined whether AD-NPC-derived neurons would exhibit normal features of neurons at molecular and functional levels. NPCs from both AD-iPSCs and N-iPSCs could spontaneously differentiate into glia or neurons of various types after withdrawal of bFGF from the NPC medium (Figure 1h and Supplementary Figure 2), suggesting that NPCs of both types had a potential to differentiate into major neural cell types. Moreover, there was no observable difference in electrophysiology properties among the neurons from AD-NPCs and N-NPCs (Figure 1i-1k).
