Nrf2 mRNA & protein expressions in the Nrf2 silenced and over expressed cell models were found to be declined or improved. Twenty-four hours of time-point was chosen in the current study because longer liposome effect on cells causes heavier hepatocytes damage, and the Nrf2 silenced and over expressed results almost the same in 24 and 48 h. The variation trend of GSTA1 mRNA relative expression was similar as Nrf2 in Figure 4. This result indirectly indicated that Nrf2 gene silencing and over expression in Nrf2 silenced and over-expressed cell models were satisfactorily used.
