a more physiologically relevant model of alcohol exposure, which allows for assessment of embryonic neurogenesis on particular cellular phenotypes. While acute in vivo alcohol exposure and subsequent gene expression analysis has been performed in either whole embryo or whole brain extracts using relatively high doses of alcohol (Aoto, Shikata, Higashiyama, Shiota, & Motoyama, 2008; Chen et al., 2013; Da Lee et al., 2004; Du & Hamre, 2003; Green et al., 2007; Hard, Abdolell, Robinson, & Koren, 2005; Zhou, 2012; Zhou et al., 2011), we present findings using a chronic moderate alcohol exposure on neural progenitor cells. Our results suggest that prenatal alcohol exposure imparts significant alterations in genes responsible for embryonic neurogenesis; thus, this ex vivo model may be useful in the transition from in vitro to in vivo studies for assessing fetal programming.
