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Chunk #39 — MATERIALS AND METHODS — Analysis of RNA and protein expression

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Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.
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Quantitative real-time PCR (qPCR), western blot, flow cytometry and immunofluorescence were performed according to standard protocols as previously described (Bertero et al., 2015). Details, including the primer sequences and antibodies used, are provided in the supplementary Materials and Methods.