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Chunk #16 — Materials and Methods — Genotyping

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Initial evidence that OPRM1 genotype moderates ventral and dorsal striatum functional connectivity during alcohol cues.
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Saliva samples were collected using Oragene saliva collection kits (Kanata, Ontario, Canada) and sent to the UCLA Genotyping and Sequencing Core for genotyping. Polymerase chain reaction was performed on Applied Biosystems (Carlsbad, CA) dual block PCR thermal cyclers. Single-nucleotide polymorphisms were run on an AB 7900HT Fast Real-Time PCR System and analyzed using the Sequence Detection Systems software version 2.3. Genotypes were automatically scored by the allele calling software, and each genotype was verified by visual inspection.