We evaluated recovery of individual TAZ-modified clones. After transfection with gRNA and HDR donor, cells were plated at low density and treated with Dox. Colonies were then picked and genotyped by DNA sequencing. Out of 42 clones sequenced, 13 (31%) contained an indel and 16 (38%) contained the donor-programmed sequence variant (Fig. 2c–d). The efficiency of our strategy and protocol has been further tested in a different human embryonic stem cell line and at different loci, with HDR rates of ~20–35% and NHEJ rates of ~50% (Suppl. Fig. 1).
