for e02539 exhibited maternal-effect lethality and increased spontaneous melanization, detected as discrete dark spots through the cuticle. These phenotypes are consistent with e02539 causing a strong loss-of-function for Spn27A (De Gregorio et al., 2002; Ligoxygakis et al., 2002). Spn27Ae02539 homozygotes were viable and had no gross morphological or behavioral deficits. Acute ethanol exposure resulted in reduced Dist (Fig. 6C,D,F). However, the ability to develop rapid tolerance was unaffected (Fig. 6E,G). These data indicate that Spn27A regulates acute responding to ethanol exposure.
