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Chunk #23 — METHODS — Microscopy and cell counting

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Notch and EGFR pathway interaction regulates neural stem cell number and self-renewal.
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and tissue sections were matched across samples. The analysis of the SVZ was performed at different anterior-posterior and dorso-ventral levels of the lateral ventricle. An average of 15–20 sections was quantifiedusing unbiased stereological morphometric analysis for the SVZ to obtain an estimate of the total number of positive cells. Then, percentages of cells expressing different antigens were estimated by scoring the number of cells double-labeled with the marker in question. In acute SVZ dissociated cells, 3 coverslips and 8–10 microscopic fields/coverslip were counted from 3 separate cultures. Statistical analysis was performed by unpaired t-test.