Cer1 and T transcription in Bptf depleted cells upon Smad2/3 activation with TGF-β1 (Figure S24F). We also used the human breast cancer cell line MCF10CA1 in similar assays to determine if BPTF could play a role in Smad signaling in humans [56]. In these experiments we used BPTF siRNAs which were effective in knocking down protein expression in MCF10CA1 cells after 2 days of culture (Figure S24G). We then stimulated the MCF10CA1 cells with TGF-β1 for 1 hour. We observed a significant reduction of PAI-1 but not SMAD7 induction suggesting that, as in the mouse, BPTF could regulate a subset of Smad responsive genes in humans (Figure S24H).
