fixative overnight with one brain in each vial. The following day, the brains were put into 70% EtOH where they sat, at room temperature, until they were embedded in paraffin. Immediately before embedding, the brains were cut at midline into two hemispheres, dehydrated/defatted in an ethanol–xylene series and placed in 64°C paraffin over night. The following day, brains were transferred twice into fresh paraffin. Brains were then embedded in a mold and cooled for sectioning. Each embedded half brain was serially sectioned in the sagittal plane at 8 µm and every 10th section was mounted on Superfrost/Plus slides. Slides were allowed to dry at 37°C overnight.
