are not always consistent across studies. This further complicates the bioinformatics predictions of the functional impact of the mirSNPs. Thus, when using this assay, the cell line must be carefully chosen to reflect the cell type of interest regarding the central biological hypothesis of the study. Since studies have shown that mirSNPs affect a wide variety of biological processes such as cancer, neurodegenerative disorders, infectious diseases, cardiovascular disease, and metabolic disorders (Bhattacharya et al., 2014), the in vitro model for testing the mirSNPs is an important consideration.
