both at room temperature. Finally, slides were mounted with SlowFade Gold mounting solution containing DAPI (Thermo Fisher Scientific). M-FISH images were visualized on a Zeiss AxioImager D1 fluorescent microscope equipped with narrow band-pass filters for DAPI, DEAC, FITC, Cy3, Texas Red and Cy5 fluorescence and an ORCA-EA CCD camera (Hamamatsu). M-FISH digital images were captured using the SmartCapture software (Digital Scientific UK), and processed using the SmartType Karyotyper software (Digital Scientific UK). Thirty metaphases for each sample were karyotyped by M-FISH.
