expression were low. AMPA receptors (GRIA2, GRIA4) were expressed in Cit+ cells at D54 and D100 (Akgul and McBain, 2016). There was low or no expression of PVALB, VIP or HTR3A, indicating that these interneuron subtypes were not produced. Raw expression levels from pooled progenitors and neurons (TPM), as well as p-values (Mann-Whitney, BH FDR correction) indicating whether expression differences between progenitors and neurons are significant, are provided in Table S1. Taken together, these data indicate that the current protocol generates MGE-like progenitors and interneurons that express the appropriate genes for specification, migration and function of GABAergic interneurons.
