We detected VGluT2 (as a glutamatergic neuron marker)-positive staining in both eDRG and aDRG NSCs (Fig. 4A, B). VGluT2 immunostaining was seen along the fibers and soma of aDRG and eDRG NSCs. Although we expected VGluT2 immunostaining to be localized in or near terminals, distribution in the soma, and along the processes suggests VGluT2 is transported to the terminal following synthesis. Transient receptor potential TrpV1 immunostaining (nociceptive, capsaicin-sensitive sensory neuron marker) (4,15,30,38) was found in differentiated eDRG and aDRG NSCs (Fig. 4C, D). Another population of eDRG and aDRG NSCs expressed pNF200 (marker for capsaicin-insensitive, myelinated DRG neurons) (Fig. 4E, F). These markers demonstrated multiple phenotypes of sensory neurons.
