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Chunk #66 — Methods — Bioinformatics Tools

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Dissection of a QTL hotspot on mouse distal chromosome 1 that modulates neurobehavioral phenotypes and gene expression.
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Classical QTLs counts are based on the April 2008 version of Mouse Genome Informatics (MGI: www.informatics.jax.org) [113]. Search for tRNAs was done using tRNAscan-SE 1.21 (http://lowelab.ucsc.edu/tRNAscan-SE/) [65]. GO analysis was done using the analytical tool DAVID 2007 (http://david.abcc.ncifcrf.gov/) [114]. Overrepresented GO terms were identified and statistical significance of enrichment was calculated using a modified Fisher's Exact Test or EASE score [115]. We used the Allen Brain Atlas to analyze expression pattern in the brain of young C57BL/6J male mice (www.brain-map.org) [85],[116].