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Chunk #29 — Results — In Vivo Assay: Differentiation of aDRG NSCs Following Transplantation

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Retentive multipotency of adult dorsal root ganglia stem cells.
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Two-weeks after transplantation, RFP+ aDRG NSCs were evident within the lesion area and up to 3 cm rostral and 1 cm caudal to the injury site. Most of the aDRG NSCs were found near the central canal or within the dorsal columns, specifically in the gracile fasciculus. A few aDRG NSCs scattered in the ventromedial funiculus in the white matter and within the gray matter. At the lesion site, many RFP+ aDRG NSCs were found but there was little colocalization with nestin (Fig. 6A–C). In the white matter, approximately one third of RFP+ aDRG NSCs in the dorsal columns and in the proprio-spinal and corticospinal tracts have neuronal-like morphology; the presence of β-III-tubulin/RFP+ cells (Fig. 6D–F) revealed that many differentiated into neural-like cells. β-III-Tubulin/RFP+ cells were located near the central canal, dorsal and ventral horn in the gray matter. RFP+ aDRG NSCs also expressed RNA binding protein Hu, but only a few were GFAP/RFP+ (Fig. 6G–I). Table 3 shows a summary of in vivo results. In examining sensory differentiation, we found a population of sensory neurons expressed pNF200, colocalized with