not only at the amino acid sequence level, but also within their RNA sequence [160]. Moreover, given that many gene knockout studies concomitantly delete potential sources of regulatory ncRNAs such as introns (given that many miRNAs and all snoRNAs are sourced from introns) and antisense transcribed sequences, all aspects of the observed phenotypes cannot be unequivocally or solely ascribed to the loss of the protein without complementation studies or more precise deletions that are rarely done for reasons of technical difficulty.
