derived cancer cell line) cells. In this unsupervised analysis, we find that the AML leukemic stem cells are more similar to LMPPs, while the AML blasts are more similar to the monocytes. In addition, we also observe that patient 1 (AML blast 1) maintains a more stem-like state when compared to patient 2 (AML blast 2) as anticipated from alternate analyses of these cells16. By visualizing the cell-specific Z-scores layered on this projection, we identify putative TFs that may promote the stem-like versus differentiated leukemia phenotype; for example, the master-regulators of myeloid cell development SPI1 (PU.1) and CEBPA17 appear as the most differential motifs between AML leukemic stem cells (LSCs) and blasts (Fig. 2b–c).
