The Stanley Medical Research Institute (SMRI) provided genomic DNA and postmortem brain tissue samples from dorsolateral prefrontal cortex (Brodmann’s area 46) from 35 individuals with schizophrenia (SCH) and 35 controls (CON). Exclusion criteria for these samples included: (1) significant structural brain pathology, (2) history of pre-existing central nervous system disease, (3) poor RNA quality, (4) documented IQ < 70, (5) age less than 30 and (6) substance abuse within one year of death46. RNA was isolated using the Ambion miRvana kit (Applied Biosystems, Foster City, CA) according to manufacturer’s instructions. We obtained poor yields of RNA from 1 CON sample, which was excluded from further study, leaving 35 SCH and 34 CON samples.
