findings in striatum from individuals with OUD5. With single nuclei resolution, we find the elevation of neuroinflammatory pathways related to microglial activation in OUD is likely due to transcriptional alterations within microglia, rather than pronounced changes in the number of striatal microglia in individuals with OUD (FDR = 0.48, linear mixed effect regression for differential abundance; Fig. 1f; Supplementary Data 1–S2).
