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Chunk #17 — Materials and Methods — Statistical analysis

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Fetal alcohol exposure alters proopiomelanocortin gene expression and hypothalamic-pituitary-adrenal axis function via increasing MeCP2 expression in the hypothalamus.
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The data shown in the figures are mean ± SEM. The significant differences between different treatment groups were assessed with one-way analysis of variance (ANOVA) with post-hoc analysis using the Newman Keuls post-test. Two-way ANOVA with Bonferroni post-hoc test was used to assess the differences between the groups of ACTH and corticosterone values. P<0.05 was considered significant.