Single-plane images of wild-type and Zbtb16 knockout cells stained with DAPI, anti-ASC and anti-SUMO antibodies were captured by confocal microscopy. The co-localisation score was calculated using the GcoPS tool in ICY65 using a threshold with constant intensity cut off on the DAPI/nuclear channel as a mask for calculating colocalization in the nucleus. The Pearson’s coefficient (colocalization) was calculated using the nuclear or H-K-means clustering (intensity cut-off: 100, clusters: 9, min/max size 100/1000) of either the DAPI stain or SUMO antibody (intensity cut-off: 80, clusters 9, min/max size 100/300). The data is graphed as the mean Pearson’s coefficient per individual image series with SEM with each image containing up to 10 cells.
