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Chunk #65 — Methods — Statistical analyses and reproducibility

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DALRD3 encodes a protein mutated in epileptic encephalopathy that targets arginine tRNAs for 3-methylcytosine modification.
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All statistics and graphs were performed and generated using GraphPad Prism software. Where applicable, error bars represent the standard deviation. Statistical tests and the number of times each experiment was repeated are stated in each figure legend. For primer extension assays, statistical analyses of the quantification were performed using one-way ANOVA and significance calculated using Tukey’s multiple comparison test. For the codon reporter assays, statistical analyses for significance was calculated a two-tailed, unpaired students t-test (t = 1.831, df = 4).