Elevated plus maze. Each mouse was placed in the center of the maze facing an open arm of the plus maze which has been previously described (Hamre et al. 2007). Mice were given 5 min to explore the maze. Both the amount of time in the various arms and the number of entries into the arms were recorded. The amount of time provides a measure of the degree of anxiety while the number of entries provides a measure of the activity level and insures that mice entered more than one arm. In addition, the amount of time in the middle of the maze was recorded. The percentage of entries into the open and closed arms were computed and recorded.
