Images were processed using ImageJ software (NIH) by researchers blind to experimental condition. Measurements consisted of particle counts and size for β-amyloid (Aβ), EEA1, and transferrin immunoreactivity, as well as signal intensity for pTau. Particle count and size were measured using a macro that converted the desired image channel into grayscale with automatic thresholding, inverting, and calling up the Analyze Particle measurement tool. Particle counts were binned by size range. Images were decoded and plotted as fold increase compared to control. For pTau immunoreactivity, the mean intensity of the entire image was measured and plotted as fold increase compared to control.
