with rabbit anti-GIRK2 (1:200 in blocking buffer; Alomone) for 2 hrs. Following rinses in 1X DPBS, cells were incubated with fluorescent secondary antibodies, anti-mouse Alexa-647 and anti-rabbit Alexa-488 (1:300; Invitrogen) for 1 hr in the dark. Cells were rinsed with 1X DPBS, mounted on microscope slides using Progold anti-fading reagent (Invitrogen), and both fluorophores imaged with a Leica TSC SP2 AOBS laser confocal microscope.
