We used electrophysiology and calcium transient imaging to measure spontaneous neuronal activity (Fig. 3D–K; SI Fig. 10). SCZD hiPSC neurons showed normal transient inward sodium currents and sustained outward potassium currents in response to membrane depolarizations (Fig. 3D), action potentials to somatic current injections, (Fig. 3E), excitatory postsynaptic currents (EPSCs) (Fig. 3F) and inhibitory postsynaptic currents (IPSCs) (Fig. 3G). The amplitude and rate of spontaneous calcium transients were unaffected (Fig. 3H–J; SI Fig. 10A–D) and there was no difference in synchronicity of spontaneous calcium transients (Fig. 3K; SI Fig. 10E–G).
