Daily ethanol consumption by mice in both the Chronic and CI treatment groups increased steadily during the first two weeks, then remained fairly stable for the remainder of the study (Figure 1A). During the last 10 days of ethanol exposure, mice in the CI group consumed more ethanol than those in the Chronic group (CI, n = 11, mean = 16 g/kg/day; Chronic, n = 10, mean = 13 g/kg/day; student’s t test, p = 0.047). Mice in the DID paradigm consumed greater quantities of ethanol during the 4 hour exposures than the 2 hour exposures but did not reach the total consumption levels attained in the other ethanol treatment paradigms. These results are consistent with previous publications [31], [45]. In all three models, mice increased their consumption of ethanol over time as indicated by a significant difference between the first and last four sessions (Figure 1B). (Percent increase and paired t test, Chronic, 24% increase, p = 0.05; CI, 59% increase, p = 6.4E–04; DID, 46% increase, p = 1.4E–04).
